KEY PUBLICATIONS
See PubMed for a complete list of publications.
*Equal contribution **co-corresponding authors
Interplay between Two Paralogous Human Silencing Hub (HuSH) Complexes in Regulating LINE-1 Element Silencing
Zena D. Jensvold, Anna E. Christenson, Julia R. Flood, Peter W. Lewis
H3 K27M and EZHIP impede H3K27-methylation spreading by inhibiting allosterically stimulated PRC2.
Siddhant U. Jain, Andrew Q. Rashoff *, Samuel D. Krabbenhoft *, Dominik Hoelper, Truman J. Do, Tyler J. Gibson, Stefan M Lundgren, Eliana R. Bondra, Shriya Deshmukh, Ashot S. Harutyunyan, Nikoleta Juretic, Nada Jabado, Melissa M. Harrison, Peter W. Lewis.
H3.3 G34W Promotes Growth and Impedes Differentiation of Osteoblast-Like Mesenchymal Progenitors in Giant Cell Tumor of Bone
Sima Khazaei*, Nicolas De Jay*, Shriya Deshmukh*, Liam D Hendrikse*, Wajih Jawhar, Carol C L Chen, Leonie G Mikael, Damien Faury, Dylan M Marchione, Joel Lanoix, Éric Bonneil, Takeaki Ishii, Siddhant U Jain, Kateryna Rossokhata, Tianna S Sihota, Robert Eveleigh, Véronique Lisi, Ashot S Harutyunyan, Sungmi Jung, Jason Karamchandani, Brendan C Dickson, Robert Turcotte, Jay S Wunder, Pierre Thibault, Peter W Lewis, Benjamin A Garcia, Stephen C Mack, Michael D Taylor, Livia Garzia, Claudia L Kleinman, Nada Jabado
Histone H3.3 G34 mutations promote aberrant PRC2 activity and drive tumor progression.
Siddhant U. Jain, Sima Khazaei, Dylan M. Marchione, Stefan M. Lundgren, Xiaoshi Wang, Daniel N. Weinberg, Shriya Deshmukh, Nikoleta Juretic, Chao Lu, C. David Allis, Benjamin A Garcia, Nada Jabado, Peter W. Lewis.
Pervasive H3K27 Acetylation Leads to ERV Expression and a Therapeutic Vulnerability in H3K27M Gliomas
Brian Krug, Nicolas De Jay, Ashot S Harutyunyan, Shriya Deshmukh, Dylan M Marchione, Paul Guilhamon, Kelsey C Bertrand, Leonie G Mikael, Melissa K McConechy, Carol C L Chen, Sima Khazaei, Robert F Koncar, Sameer Agnihotri, Damien Faury, Benjamin Ellezam, Alexander G Weil, Josie Ursini-Siegel , Daniel D De Carvalho, Peter B Dirks, Peter W Lewis, Paolo Salomoni, Mathieu Lupien, Cheryl Arrowsmith, Paul F Lasko , Benjamin A Garcia, Claudia L Kleinman, Nada Jabado, Stephen C Mack
PFA ependymoma-associated protein EZHIP inhibits PRC2 activity through a H3 K27M-like mechanism
Siddhant U Jain, Truman J Do, Peder J Lund, Andrew Q Rashoff, Katharine L Diehl, Marcin Cieslik, Andrea Bajic, Nikoleta Juretic, Shriya Deshmukh, Sriram Venneti, Tom W Muir, Benjamin A Garcia, Nada Jabado, Peter W Lewis.
Nature Communications 2019 May 13;10(1):2146
Article Coverage: Nature News and Views
Histone H3 tail binds a unique sensing pocket in EZH2 to activate the PRC2 methyltransferase
Krupa S. Jani, Siddhant U. Jain, Eva J. Ge, Katharine L. Diehl, Stefan M. Lundgren, Manuel M. Müller, Peter W. Lewis, Tom W. Muir.
PNAS 2019 Apr 23;116(17):8295-8300
H3K27M induces defective chromatin spread of PRC2-mediated repressive H3K27me2/me3 and is essential for glioma tumorigenesis
Ashot S. Harutyunyan*, Brian Krug*, Haifen Chen, Simon Papillon-Cavanagh, Michele Zeinieh, Nicolas De Jay, Shriya Deshmukh, Carol C.L. Chen, Jad Belle, Leonie G. Mikael, Dylan M. Marchione, Rui Li, Hamid Nikbakht, Bo Hu, Gael Cagnone, Warren A. Cheung, Abdulshakour Mohammadnia, Denise Bechet, Damien Faury, Melissa K McConechy, Manav Pathania, Siddhant U. Jain, Benjamin Ellezam, Alexander G. Weil, Alexandre Montpetit, Paolo Salomoni, Tomi Pastinen, Chao Lu, Peter W. Lewis, Benjamin A. Garcia Claudia L. Kleinman, Nada Jabado**, Jacek Majewski**
Nature Communications 2019 Mar 19;10(1):1262
S-adenosylmethionine biosynthesis is a targetable metabolic vulnerability of cancer stem cells
Elena Strekalova, Dmitry Malin, Erin M. M. Weisenhorn, Jason D. Russell, Dominik Hoelper, Aayushi Jain, Joshua J. Coon, Peter W. Lewis, Vincent L. Cryns.
Brest Cancer Res. Treat. 2019 May;175(1):39-50
ATRX, DAXX or MEN1 mutant pancreatic neuroendocrine tumors are a distinct alpha-cell signature subgroup
Chang S Chan*, Saurabh V Laddha*, Peter W Lewis, Matthew S Koletsky, Kenneth Robzyk, Edaise Da Silva, Paula J Torres, Brian R Untch, Janet Li, Promita Bose, Timothy A Chan, David S Klimstra, C David Allis, Laura H Tang
Nature Communications 2018 Oct 12;9(1):4158
Recognition of cancer mutations in histone H3K36 by epigenetic writers and readers
Brianna J Klein, Krzysztof Krajewski, Susana Restrepo, Peter W Lewis, Brian D Strahl, Tatiana G Kutateladze
Structural and mechanistic insights into ATRX-dependent and -independent functions of the histone chaperone DAXX
Dominik Hoelper, Hongda Huang, Aayushi Y Jain, Dinshaw J Patel, Peter W Lewis.
Impaired H3K36 methylation defines a subset of head and neck squamous cell carcinomas
Simon Papillon-Cavanagh*, Chao Lu*, Tenzin Gayden, Leonie G Mikael, Denise Bechet, Christina Karamboulas, Laurie Ailles, Jason Karamchandani, Dylan M Marchione, Benjamin A Garcia, Ilan Weinreb, David Goldstein, Peter W Lewis, Octavia Maria Dancu, Sandeep Dhaliwal, William Stecho, Christopher J Howlett, Joe S Mymryk, John W Barrett, Anthony C Nichols, C David Allis, Jacek Majewski, Nada Jabado
Nature Genetics 2017 Feb;49(2):180-185
S-adenosyl methionine is necessary for inhibition of the methyltransferase G9a by the lysine 9 to methionine mutation on histone H3
Hariharan Jayaram*, Dominik Hoelper*, Siddhant U. Jain, Nico Cantone, Stefan M. Lundgren, Florence Poy, C. David Allis, Richard Cummings, Steven Bellon, and Peter W. Lewis
Histone H3K36 mutations promote sarcomagenesis through altered histone methylation landscape
Chao Lu, Siddhant U. Jain, Dominik Hoelper, Denise Bechet, Rosalynn C. Molden, Leili Ran, Devan Murphy, Sriram Venneti, Meera Hameed, Bruce R. Pawel, Jay S. Wunder, Brendan C. Dickson, Stefan M. Lundgren, Krupa S. Jani, Nicolas De Jay, Simon Papillon-Cavanagh, Irene L. Andrulis, Sarah L. Sawyer, David Grynspan, Robert E. Turcotte, Javad Nadaf, Somayyeh Fahiminiyah, Tom W. Muir, Jacek Majewski, Craig B. Thompson, Ping Chi, Benjamin A. Garcia, C. David Allis, Nada Jabado, Peter W. Lewis
Lowered H3K27me3 and DNA hypomethylation define poorly prognostic pediatric posterior fossa ependymomas
Jill Bayliss, Piali Mukherjee, Chao Lu, Siddhant U Jain, Chan Chung, Daniel Martinez, Benjamin Sabari, Ashley S Margol, Pooja Panwalkar, Abhijit Parolia, Melike Pekmezci, Richard C McEachin, Marcin Cieslik, Benita Tamrazi, Benjamin A Garcia, Gaspare La Rocca, Mariarita Santi, Peter W Lewis, Cynthia Hawkins, Ari Melnick, C David Allis, Craig B Thompson, Arul M Chinnaiyan, Alexander R Judkins, Sriram Venneti
Science Translational Medicine 2016 Nov 23;8(366):366ra161
Histone chaperone ASF1B promotes human β-cell proliferation via recruitment of histone H3.3
Pradyut K Paul, Mary E Rabaglia, Chen-Yu Wang, Donald S Stapleton, Ning Leng, Christina Kendziorski, Peter W Lewis, Mark P Keller, Alan D Attie
Cell Cycle 2016 Dec;15(23):3191-3202
Targeted Histone Peptides: Insights into the Spatial Regulation of the Methyltransferase PRC2 by using a Surrogate of Heterotypic Chromatin
Zachary Z Brown, Manuel M Müller, Ha Eun Kong, Peter W Lewis, Tom W Muir
Angewandte Chemie 2015 May 26;54(22):6457-61
ATRX tolerates activity-dependent histone H3 methyl/phos switching to maintain repetitive element silencing in neurons
Kyung-Min Noh, Ian Maze, Dan Zhao, Bin Xiang, Wendy Wenderski, Peter W Lewis, Li Shen, Haitao Li, C David Allis
PNAS 2015 Jun 2;112(22):6820-7
Use of human embryonic stem cells to model pediatric gliomas with H3.3K27M histone mutation
Kosuke Funato, Tamara Major, Peter W. Lewis, C. David Allis, Viviane Tabar
Science 2014 Dec 19;346(6216):1529-33
Strategy for "detoxification" of a cancer-derived histone mutant based on mapping its interaction with the methyltransferase PRC2
Zachary Z Brown*, Manuel M Müller*, Siddhant U Jain, C David Allis, Peter W Lewis, Tom W Muir
J. Am. Chem. Soc. 2014 Oct 1;136(39):13498-501
A sensitive and specific histopathologic prognostic marker for H3F3A K27M mutant pediatric glioblastomas
Sriram Venneti, Mariarita Santi, Michelle Madden Felicella, Dmitry Yarilin, Joanna J Phillips, Lisa M Sullivan, Daniel Martinez, Arie Perry, Peter W Lewis, Craig B Thompson, Alexander R Judkins
Acta Neuropathologica 2014 Nov;128(5):743-53
Specific detection of methionine 27 mutation in histone 3 variants (H3K27M) in fixed tissue from high-grade astrocytomas
Denise Bechet, Gerrit G H Gielen, Andrey Korshunov, Stefan M Pfister, Caterina Rousso, Damien Faury, Pierre-Olivier Fiset, Naciba Benlimane, Peter W Lewis, Chao Lu, C David Allis, Mark W Kieran, Keith L Ligon, Torsten Pietsch, Benjamin Ellezam, Steffen Albrecht, Nada Jabado
Acta Neuropathologica 2014 Nov;128(5):733-41
Strategy for "detoxification" of a cancer-derived histone mutant based on mapping its interaction with the methyltransferase PRC2
Zachary Z Brown*, Manuel M Müller*, Siddhant U Jain, C David Allis, Peter W Lewis, Tom W Muir
JACS 2014 Oct 1;136(39):13498-501
KDM4A lysine demethylase induces site-specific copy gain and rereplication of regions amplified in tumors
Joshua C Black, Amity L Manning, Capucine Van Rechem, Jaegil Kim, Brendon Ladd, Juok Cho, Cristiana M Pineda, Nancy Murphy, Danette L Daniels, Cristina Montagna, Peter W Lewis, Kimberly Glass, C David Allis, Nicholas J Dyson, Gad Getz, Johnathan R Whetstine
Inhibition of PRC2 activity by a gain-of-function H3 mutation found in pediatric glioblastoma
Peter W Lewis, Manuel M Müller, Matthew S Koletsky, Francisco Cordero, Shu Lin, Laura A Banaszynski, Benjamin A Garcia, Tom W Muir, Oren J Becher, C David Allis
Science 2013 17;340(6134):857-61
Poisoning the "histone code" in pediatric gliomagenesis
Peter W Lewis, C David Allis
DAXX envelops a histone H3.3-H4 dimer for H3.3-specific recognition
Simon J Elsässer, Hongda Huang, Peter W Lewis, Jason W Chin, C David Allis, Dinshaw J Patel
Nature 2012 Nov 22;491(7425):560-5
Daxx is an H3.3-specific histone chaperone and cooperates with ATRX in replication-independent chromatin assembly at telomeres
Peter W Lewis, Simon J Elsaesser, Kyung-Min Noh, Sonja C Stadler, C David Allis
Histone variants in metazoan development
Laura A Banaszynski, C David Allis, Peter W Lewis
Developmental Cell 2010 Nov 16;19(5):662-74
Distinct factors control histone variant H3.3 localization at specific genomic regions
Aaron D Goldberg, Laura A Banaszynski*, Kyung-Min Noh*, Peter W Lewis, Simon J Elsaesser, Sonja Stadler, Scott Dewell, Martin Law, Xingyi Guo, Xuan Li, Duancheng Wen, Ariane Chapgier, Russell C DeKelver, Jeffrey C Miller, Ya-Li Lee, Elizabeth A Boydston, Michael C Holmes, Philip D Gregory, John M Greally, Shahin Rafii, Chingwen Yang, Peter J Scambler, David Garrick, Richard J Gibbons, Douglas R Higgs, Ileana M Cristea, Fyodor D Urnov, Deyou Zheng, C David Allis